How are cDNAs made?
Answer: Reverse Transcriptase.
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Cloning
- Basic principles of Genetic Knockdown?
- An epitope tagged protein can be purified through ____ & _____.
- Describe a Fusion Protein: Epitope Tag
- In a yeast two hybrid system the ________ is not delivered by a plasmid.
- What is the purpose of a Fusion Protein?
- Describe a Fusion Protein: GFP Tag?
- _______ libraries have to be used when studying promoter regions.
- Component of a Reporter Assay Plasmid?
- What is the purpose of a Reporter Assay?
- What is the natural function of Restriction Enzymes in bacteria ?
- How do you select for cells that have recombinant plasmids?
- How do you select for cells that have taken up a plasmid?
- What is Blue/White selection?
- If you insert a gene inside a tetracycline region, what will be the drug resistance of the resulting plasmid?
- What 2 methods are used to make cell competent to take up DNA (transfection)? How do they do it?
- How do we ensure that the gene of interest is inserted into a plasmid?
- What can cDNA be used for?
- Why do plasmids have to be pre-spliced?
- How do we create a PCR product we can insert into a plasmid?
- How can human insulin be made in bacteria?
- T/F: Only endogenous genes of bacteria can be replicated.
- Why is insulin no longer made by using the pancreas of animals?
- What are the advantages of Polylinkers?
- What are other common features of vectors?