What 2 methods are used to make cell competent to take up DNA (transfection)? How do they do it? Cloning What 2 methods are used to make cell competent to take up DNA (transfection)? How do they do it? Answer: Treatment with CaCl2, Electroporation. Make membrane permeable to plasmid. Learn More : Share this Share on FacebookTweet on TwitterPlus on Google+