What is Blue/White selection?
Answer:
Plasmid: Antibiotic Marker + LacZ.
Grow cells in a plate with X-Gal + Antibiotic.
Plasmid ligates to itself: resistant, blue.
Plasmid + insert: resistant, white.
What is Blue/White selection?
Answer:
Plasmid: Antibiotic Marker + LacZ.
Grow cells in a plate with X-Gal + Antibiotic.
Plasmid ligates to itself: resistant, blue.
Plasmid + insert: resistant, white.